胃癌术后硫酸性肠化切缘P^（53）和ras　P^（21）蛋白的表达及其意义

P￣(53)and ras P￣(21) Protein expression at the resected margins of sulfomucin type of intestinal metaplasia and its significance in post-operative patients with carcinoma of stomach

目的探讨硫酸性肠化切缘Ｐ５３和ｒａｓＰ２１蛋白的表达，进一步从分子生物学水平证实硫酸性肠化与胃癌术后切缘复发的关系。方法应用流式细胞术和细胞免疫荧光染色技术，对１８例硫酸性和１７例非硫酸性肠化切缘粘膜上皮细胞的Ｐ５３和ｒａｓＰ２１蛋白的表达量进行了定量研究．结果硫酸性肠化切缘上皮的Ｐ５３和ｒａｓｐ２１蛋白的表达量显著高于非硫酸性肠化切缘和正常粘膜上皮，而低于胃癌细胞．结论硫酸性肠化切缘的ｐ５３和ｒａｓＰ２１蛋白有过量表达，可能与胃癌术后复发有关。

Objective To research for P53 and ras P21 protein expression at resected margins of sulfomucin type intestinal metaplasia (SIM), and to determine the relationship between SIM and local recurrence after operation for gastric carcinoma.Method P53 and ras P21 expression of the resected margins of SIM in 18 cases and 17 cases of N-SIM were quantitatively studied in post- operative patients with carcinoma of stomach by flow cytometry and immunoflurecence method.Results P53 and ras P21 protein expression in the resected margins of SIM were higher than those in N- SIM and normal gastric mucosa cells, but lower than those of cells, of gastric carcinoma.Conclusion The resected margins of SIM have high expression of P53 and ras P21, and may related to local recurrence in post operative patients with carcinoma of stomach.