^(12)C^(6+)离子诱导TNFα基因在SMMC-7721细胞中的表达

Overexpression of TNFα gene in SMMC-7721 cells irradiated with ^(12)C^(6+) ion

目的用Polyfect转染试剂将重组质粒pEgr-TNFα转染人肝癌细胞SMMC-7721,研究该重组质粒经12C6+离子作用后在细胞中的表达。方法用ELISA方法检测TNFα的蛋白表达,用RT-PCR方法检测TNFα转录水平的变化。结果转染pEgr-TNFα后在SMMC-7721细胞中TNFα的蛋白表达量为2·06ng/ml,2Gy12C6+离子照射后,表达量增高为2·11ng/ml,明显高于未转染组和转染空载体组;RNA转录水平也明显增高,2Gy12C6+离子照射后增高更明显。结论12C6+离子联合重组质粒pEgr-TNFα,在被转染的SMMC-7721细胞中表达量明显增高。

Objective To investigate the expression of pEgr-TNFα in SMMC-7721 cells, which were transfected by polyfect transfection reagent and irradiated with carbon ion ^12 C^6＋. ion. Methods The protein expression of TNFa was measured with ELISA, its transcription level was determined by RT-PCR. Results The expression of TNFa was measured to be 2.06 ng/ml when transfected by pEgr-TNFa, and increased to 2.11 ng/ml with irradiation of 2 Gy ^12 C^6＋ , which was significantly higher than that of the control group or the vector group. The transcription level of TNFa was increased with the transfection; more significant higher level was observed with irradiation of 2 Gy ^12 C^6＋ . Conclusion Carbon ions combined with pEgr-TNFa can significantly increase TNFa expression level in SMMC-7721 cells.