中药对大鼠辐射损伤防护作用的实验研究

Study on preventive and therapeutic effect of Chinese medicinal herbal extracts on rat with bone marrow injury induced by radiation exposure

目的探讨中药当归、川芎、黄芪、丹参提取液对大鼠放射性骨髓损伤的防护作用。方法雄性SD大鼠60只,随机分为正常对照组、模型组、中药组,每组6只,在相同条件下饲养2周,模型组和中药组大鼠给予6·0Gy60Coγ射线一次性全身照射,继续饲养1周处死全部动物并取材。用骨髓细胞计数法计数骨髓有核细胞数,用Westernblot法测定血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)、血小板衍生生长因子(ptateletderivedgrowthfactor,PDGF)的蛋白含量。结果与正常对照组比较,模型组大鼠的骨髓有核细胞数、VEGF、PDGF蛋白含量明显减少(P<0·01),与模型组比较,中药组大鼠的骨髓有核细胞数、VEGF、PDGF蛋白含量明显增加(P<0·01或P<0·05)。结论中药当归、黄芪、川芎、丹参对大鼠放射性骨髓损伤有一定防护作用。

Objective To examine the effect of Chinese medicinal herbal extracts, Danggni （Radix angelicae sinertsis ）, Chuanxiong （ Rhizoma chuanxiong ）, Huangqi （ Radix astragali ）, and Danshen （ Radix salviae miltiorrhizae） on rats with bone marrow injury induced with whole-body gamma-ray exposure. Methods Sixty male rats were randomly divided into three groups, control group, model group （ irradiation only with no administration of the extracts）, and drug treatment group （ irradiation and administration of Chinese medicinal herbal extracts）. Rats were irradiated with 6 Gy cobolt-60 gamma rays after administration of the extracts for two weeks. The number of marrow nucleate cells was counted, and VEGF and PDGF expression were measured with Western blot method on the 7th day since the irradiation. Results Bone marrow nucleate cells and VEGF and PDGF expression in bone marrow cells in the model group were significantly lower than those in the control group （ P 〈 0.01 ）, and these values in the drug treatment group were significantly higher than those in the model group （ P 〈 0.01 or P 〈 0.05）. Conclusion The extracts of Chuanxiong, Danggui, Huangqi, and Danshen can be used to prevent from ration bone marrow injury in rats.