AgNOR在胃镜刷检细胞中的诊断意义

Diagnostic significance of AgNOR in gastroscope-brushing cytology

目的探讨核仁组成区蛋白（ＡｇＮＯＲ）在冒镜刷检细胞学良、恶性鉴别诊断中的价值．方法４９例胃镜刷检细胞学涂片以胶质银技术染色，依据上海方案进行ＡｇＮＯＲ观察、以Ｈ－Ｅ染色涂片及组织学切片相对照．结果随鳞状上皮病变级别增高，ＡｇＮＯＲ均数逐渐增高；腺癌细胞ＡｇＮＯＲ均数明显高于正常腺上皮细胞（Ｐ＜０．０５）；正常细胞与癌细胞ＡｇＮＯＲ数目、形态、大小及分布都有差异．应用ＡｇＮＯＲ染色观察．使细胞学与组织学的诊断符合率由原来的７５．５１％提高到８３．６７％．结论ＡｇＮＯＲ在胃镜刷检细胞学中是反映细胞增殖水平较敏感指标，在大规模细胞学普查中可能将有较大的实用价值．

Objective The purpose of this study was to evaluate the role of AgNOR in the differenting diagnosis of benign or malignanty in gastroscope-brushing cytology.Methods The smears of gastroscope-brushing cytology in 49 patients were stained with silver colloid technique. AgNOR were studied according to Shanghai sytem, and compare with HE staining smears and sections. Results The mean number of AgNOR increased in proportion to the seventy of lesion of squamous cells.The mean value of AgNOR in adenocarcinoma cell was more than that in normal glandular epithelium cell were observed (P<0.05); AgNOR in cancer cells are different from that in normal cells in amount, shape, size and distribution within nucleus.The conformance rate of cytology to histology increased from 75. 51% to 83. 67% by means of AgNOR determination. Conclusion AgNOR is a sensitive index to reflect proliferative activety of cells in gastroscope-brushing cytology, and may possesse more practical value in general surver of esophageal and gastric diseases.