脑溢安对脑出血大鼠脑内BDNF mRNA表达的影响

Effect of Naoyi-an on the expression of brain-derived neurotrophic factor mRNA in rats with intracerebral hemorrhage

目的观察脑出血模型大鼠脑内脑源性神经营养因子mRNA(BDNF mRNA)的分布以及中药脑溢安对其影响。方法通过微量注射器向苍白球内注入Ⅶ型胶原酶0·4U制作脑出血模型,用原位杂交法观察脑出血后2、6、12h及1、4、7d共6个时间点BDNF mRNA的表达变化,以阳性细胞计数作为观察指标。结果正常组、假手术组大鼠脑内皮质、海马、纹状体等处有BDNF mRNA的表达。脑出血后2h上述部位BDNFmRNA的表达增高,但血肿中心区域未见表达信号,6h后上述部位表达信号继续增强,1d后达高峰,4d后表达开始减弱,7d后其表达水平继续下降但仍高于正常,14d后BDNF mRNA阳性细胞数量及表达水平降至基础水平。两者的阳性细胞主要表达于神经元,其中在6h、1d、4d3个时间点上,两组阳性细胞计数比较有显著性差异(P<0·01)。结论脑溢安可促进脑出血后BDNF mRNA的表达。

Objective To observe the distribution of brain-derived neurotrophic factor （BDNF） mRNA in rats with intraeerebral hemorrhage （ICH） and effect of Naoyi-an （NYA） on it. Methods The ICH rat models were established by microinjecting 0. 4 ？ 1 Ⅶ collagenase into the globus pallidus to observe the expression variation of BDNF mRNA at 2, 6, 12 h, 1,4 and 7 d after ICH by in situ hybridization technique. And positive cell counts served as the observation index. Results BDNF mRNA expressed in cortex, hippocampus, striatum of normal controls and sham-operated rats. The expressions of BDNF mRNA in above-mentioned regions increased 2 h after ICH, but were not found in the hematoma region, and continuously increased in above-mentioned regions 6 h after ICH, then reached the peak after 1 d, began to decrease 4 d after ICH, and decreased continuously but were yet higher than the normal level after 7 d, and positive cells come around to the normal level in ceils quantity and expression level 14 d after ICH. Both positive ceils were mainly expressed in neurons. The BDNF mRNA expression level of NYA group were higher than that of the model group at 6 h, 1 and 4 d time point （ P 〈 0. 01 ）. Conclusions NYA could upregulated the expression of BDNF mRNA after ICH.