高能混合粒子场辐照小麦M_1代变异的SSR分析

SSR ANALYSIS ON M_1 VARIATION OF WHEAT IRRADIATED BY MIXED HIGH ENERGY PARTICLE FIELD

本试验利用北京正负电子对撞机直线加速器E2束流打靶产生高能次级混合粒子场,模拟次级宇宙射线,分别以0、109、145、195、284和560Gy剂量处理两个冬小麦品种ZY9和ZH7,并与相同剂量的60Coγ射线相比较,研究混合粒子场辐射处理对小麦幼苗生长和微卫星(SSR)标记谱带变化的影响。试验结果表明,随着混合粒子场处理剂量的增加,小麦幼苗生长受到的损伤逐渐增加。SSR标记分析结果发现,小麦B基因组的多态性位点频率占总多态性频率的46%,在3个基因组中最高,可能为“热点”突变基因组。高能混合粒子场对冬小麦幼苗的生长抑制和微卫星DNA的损伤效应大于γ射线。

Dry seeds of two winter wheat genotypes, ZY9 and ZH7, were irradiated by simulative cosmic rays of mixed highenergy particle field （shorted as CR） with different dosages of 0, 109, 145, 195, 284 and 560Gy, which was generated from E2 of LINAC of Beijing Electron Positron Collider, ^（60） Co γ-rays irradiation of the same dosage as the control. The results indicated that there were dose-effects on seedling height and root length of CR irradiation. The total rate of polymorphic loci in genome B was 46%, which was the highest in the three wheat genomes, implying B genome might be the hotspot mutation genome in wheat. The microsatellite DNA damage caused by CR was more serious than those of γ-rays in the M1 generation of wheat.