摘要
为改良水稻(Oryza sativaL.)的耐逆性,以来源于成熟种子的胚性愈伤组织为受体材料,通过农杆菌介导法将拟南芥(Arabidopsis thaliana)耐逆相关CBF1基因导入粳稻品种秀水11基因组,经GUS组织化学染色、PCR检测和Southern杂交分析验证,获得一批转基因植株。T1代检测结果显示,所转基因已遗传给后代,且大多数株系的分离比符合3∶1。试验表明,在高盐与高渗胁迫下,转基因株系较非转化对照具有显著或极显著生长优势,表现苗高负增长率较小,长出的根数较多,根长较长;经低温胁迫处理后,转基因株系的叶片相对电导率显著或极显著低于对照。这些结果证明水稻转基因株系的耐逆性得到了增强。
In order to improve stress tolerance of rice （ Oryza sativa L. ）, Agrobacterium tumefaciens strain EHAI05 carrying plasmid pCMD containing stress tolerance-related CBF1 gene from Arabidopsis thaliana was used to transform mature seedsderived embryogenic calli of japonica variety Xiushui 11. More than one hundred transgenic plants were regenerated, which were confirmed by histochemical detection of GUS activity, PCR assay and Southern hybridization analysis. The transgene was inherited to T1 progeny and Mendelian 3 ： 1 segregation ratio was observed in most transgenic lines. Under high salinity and high osmosis stresses, transgenic lines had significant （ P 〈 0.05 ） or highly significant （ P 〈 0.01 ） growth superiority compared with non-transformed control, including smaller decrease in seedling height and formation of more and longer roots.Additionally, relative electronic conductivity of low temperature-stressed leaves of transgenic lines was significantly or highly significantly lower than that of the control. These results demonstrated that stress tolerance of transgenic rice lines was enhanced.
出处
《核农学报》
CAS
CSCD
北大核心
2006年第3期169-173,共5页
Journal of Nuclear Agricultural Sciences
基金
浙江省"151人才基金"项目
关键词
水稻
根癌农杆菌
遗传转化
CBF1基因
耐逆性
rice （ Oryza sativa L. ）
Agrobacterium tumefaciens
genetic transformation
CBF1 gene
stress tolerance
