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高羊茅DREB类转录因子基因的分离及鉴定分析 被引量：20

ISOLATION AND CHARACTERIZATION OF A DREB-LIKE TRANSCRIPTION FACTOR GENE FROM TALL FESCUE
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摘要 DREB转录因子是植物中特有的与低温、高盐和干旱胁迫相关的反式作用因子。为了分离和鉴定高羊茅(Festuca arundinaceaSchreb.)DREB转录因子基因,我们构建了冷诱导(4℃)的高羊茅cDNA文库,用拟南芥DREB1A基因作探针筛选该文库得到一个DREB类基因FaDREB1。序列分析表明,FaDREB1具有一个651bp的开放阅读框和229bp的3’非编码区,推测的氨基酸序列中含有一个高度保守的EREBP/AP2结构域。酵母单杂交结果表明FaDREB1蛋白能在体外特异结合DRE元件,并具有转录激活功能。Northern杂交结果发现FaDREB1基因受低温的诱导表达,对高盐、干旱和ABA没有反应,说明FaDREB1基因在高羊茅植株对低温的应答反应中起重要作用。 DREB transcription factors are unique trans-acting factors that involved in low-temperature, high-salt and drought stress responses in plants. To isolate DREB genes from tall fescue （ Festuca arundinacea Schreb）, we constructed a cDNA library of cold （4℃） stressed F. arundinacea seedlings and identified a cDNA＇clone, FaDREB1, by screening the library with DREB1A gene of Arabidopsis as probe. The FaDREB1 has an open reading frame of 651bp with a 3＇-UTR of 229bp. The putative protein contains a highly conserved EREBP/AP2 domain. In yeast one-hybrid analysis FaDREB1 protein was specifically bound to DRE elements and activated the expression of the reporter genes of HIS3 and LacZ. Northern blotting results indicated that FaDREB1 gene obviously induced by low-temperature, but not by high-salt, drought stress and ABA, suggesting an important role of FaDREB 1 in response to cold stress in F. arundinacea.
作者 阳文龙 刘敬梅 刘强 公衍道 赵南明
机构地区 清华大学生物技术系
出处 《核农学报》 CAS CSCD 北大核心 2006年第3期187-192,共6页 Journal of Nuclear Agricultural Sciences
基金 国家"973"项目 国家"863"项目(批准号2002AA224091) 国家杰出青年基金(批准号30125005)
关键词 高羊茅 DRE元件 转录因子 胁迫 tall fescue （Festuca arundinacea Schreb） DRE transcription factor stress
分类号 S543.9 [农业科学—作物学] S688.4 [农业科学—观赏园艺]
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参考文献22

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二级参考文献48

  • 1Baker SS, Wilhelm KS, Thomashow MF. The 5′-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression. Plant Mol Biol, 1994,24:701～713
  • 2Jiang C, Iu B, Singh J. Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus. Plant Mol Biol, 1996,30:679～684
  • 3Wang H, Datla R, Georges F et al. Promoters from Kin1 and cor 6.6, two homologous Arabidopsis thaliana genes: transcriptional regulation and gene expression induced by low temperature, ABA osmoticum and dehydration. Plant Mol Biol, 1995,28:605～617
  • 4Iwasaki T, Kiyosue T, Yamaguchi-Shinozaki K et al. The dehydration-inducible rd17 (cor47) gene and its promoter region in Arabidopsis thaliana. Plant Physiol, 1997,115:1287
  • 5Stockinger EJ, Gilmour SJ, Thomashow MF. Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit. Proc Natl Acad Sci USA, 1997,94:1035～1040
  • 6Ouellet F, Vazquez-Tello A, Sarhan F. The wheat wcs120 promoter is cold-inducible in both monocotyledonous and dicotyledonous species. FEBS Lett, 1998,423:324～328
  • 7Gilmour SJ, Zarka DG, Stockinger EJ et al. Low temperature regulation of the Arabidopsis CBF family of AP2 transcriptional activators as an early step in cold-induced COR gene expression. Plant J, 1998,16(4):433～442
  • 8Medina J, Bargues M, Terol J et al. The Arabidopsis CBF gene family is composed of three genes encoding AP2 domain-containing proteins whose expression is regulated by low temperature but not by abscisic acid or dehydration. Plant Physiol, 1999,119(2): 463～470
  • 9Liu Q, Kasuga M, Sakuma Y et al. Two transcription factors, DREB1 and DREB2, with an EREBP/AP2 DNA binding domain separate two cellular signal transduction pathways in drought- and low-temperature-responsive gene expression, respectively, in Arabidopsis. Plant Cell, 1998,10(8):1391～1406
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核农学报
2006年 第3期

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