脑瘫—正常双生子卵型鉴定与病证调查分析

To Diagnose Zygosity and to Investigate and Analyse the Pathogeny and Symptom of the Twins

目的:以一对脑瘫—正常双生子的卵型鉴定与全面病因病证调查作基础,为集约地筛选形成脑瘫的主效基因作铺垫。方法:对脑瘫—正常双生子分别采用性状相似评分、指纹特征比较、血型、胎盘膜隔数和短串联重复序列(STR)等5种方法鉴别卵型。采用粗大运动功能评估(GMFM)、生活能力(ADL)、双生子四诊调查表、肾虚辨证因子、脑瘫脾肾两虚调查表等5个量表全面地评定病证特征。结果:脑瘫儿大双和正常儿小双为单卵双生子,大双GMFM和ADL评分分别为26、51·5;肾虚量表评分中脑瘫儿大双35分,正常儿小双7分;脾肾两虚量表大双肾虚评分为11分,脾虚分为14分,正常小双均为0分。讨论:对双生子卵型鉴定方法进行了全面应用分析,前瞻性的分析了以脑瘫—正常双生子为对象进行脑瘫证候分子生物学机制研究的优势。

Objective： In order to pave a way for selecting the main relative gene of resulting in cerebral palsy by investigating the pathogeny and symptom of the twin sisters that one is healthy while the other suffers from cerebral palsy and by diagnosing zygosity of tw/ns by five.means. Methods：Grading for character, comparing the fingerprint, determining blood type, counting placenta dissepiment and examining the short repeat seqence were introduced to diagnose zygosity of twins. GMFM,ADL and the zheng -hou table were introduced to assess the symptom roundly. Results：The twin belongs to monozygotic twins. The grade of GMFM and ADL is 26 and 51.5 respectively. The eider＇sister and the younger sister in the grade of the kidney deficiency is 35 and 7 respectively, at the same time, the kidney - spleen deficiency is 11 and 14 respectively while the smaller sister in this part is zero. Discusstions： To analyse completely the methods of the diagnosing zygosity. The further study of mechanism that may influence susceptibility to cerebral palsy in the level of the molecule biology is prospective by using the especial object and the efficiency cDNA microarray.