摘要
目的探讨前列腺素E2(PGE2)免疫干预对肝病大鼠创伤弧菌(vibriovulnificus,Vv)攻击后细胞因子、超氧化物歧化酶(SOD)、一氧化氮(NO)的影响,及与肺组织超微结构改变的相关性。方法将正常大鼠9只,肝病大鼠36只,分为正常大鼠Vv攻击组、肝病大鼠Vv攻击组、肝病Vv攻击后氧氟沙星等药物治疗组(简称氧氟沙星组)、肝病Vv攻击PGE2氧氟沙星等联合保护组(简称PGE2免疫干预组)和肝病大鼠对照组,用ELISA法测定IL-10、IL-1β和IL-6含量;化学比色法定量测定SOD、NO含量。取各组大鼠肺标本在电镜下观察超微结构病理改变。结果肝病大鼠较正常大鼠Vv攻击后IL-10、SOD低,而IL-1β、IL-6和NO高(P<0.05或P<0.05);PGE2免疫干预组比氧氟沙星组的IL-10、SOD高(均P<0.05),而IL-1β、IL-6和NO低(P<0.05或0.05);肺组织超微结构明显改善。结论PGE2通过上调肝病大鼠血清IL-10、SOD含量,抑制IL-1β和IL-6分泌以及降低NO的含量,使肺组织损伤得到改善。
Objective To investigate the protective effect of prostaglandin E2（PGE2） on lung tissue in rats with hepatopathy attacked by vibrio vulnificus （Vv）. Methods Nine normal rats were attacked by Vv without any treatment and served as control group. Thirty six rats with hepatopathy were divided into 4 groups： Vv attack ＋ no treatment, Vv attack ＋ ofloxacin treatment, Vv attack ＋ PGE2 with ofloxacin treatment and no Vv attack （n=9 in each group）. The serum levels of IL-10, IL-1β and IL-6 were measured by enzyme linked immunoabsorbent assay （ELISA）, serum SOD and NO levels were detected with chemistry technique, sections of lung tissue were examined under electron microscopy. Results Serum SOD and IL-10 levels of PGE2＋ ofloxacin group were significantly higher than those of ofloxaein group; while NO, IL-1β and IL-6 levels of PGE2 group were significantly lower than those of ofloxacin group （P〈0.01）. Lung injury was improved significantly after treatment with PGE2. Conclusion PGE2 may increase serum SOD and IL-10 concentrations and inhibit serum NO, IL-1β and IL-6 levels, so that treatment with PGE2 may protect lung tissue from injury induced by Vv attack.
出处
《浙江医学》
CAS
2006年第6期442-444,448,共4页
Zhejiang Medical Journal
基金
温州市科技局科研基金资助项目(S2002A121)
