培养基中糖和氮对脱毒甘薯组培苗生长的影响

Effects of sugar and nitrogen on virus-free plantlet growth of sweet potato in vitro

以脱毒甘薯的茎段为材料,研究培养基中不同糖种类、糖浓度、NH4+/NO3-及总氮量对甘薯组培苗扩繁的影响.结果表明:在MS培养基添加蔗糖或白糖,NH4+/NO3-含量为30/30(mM/mM),总氮量控制在30,60 mM时对甘薯组培扩繁有利.考虑到培养基成本,脱毒甘薯培养基以MS+食用白糖50 g/L+NH4+/NO3-30/30(mM/mM)+总氮量30 mM最为适宜.将脱毒甘薯组培苗移栽到含蛭石和草炭的营养钵中进行驯化,成活率可达95%.

The effects of different types of sugar, sugar with different concentrations,NH4^＋/NO3^-,and total nitrogen in culture medium on the micropropagation of sweet potatoes were studied with nodal cuttings of virus-free plantlet. The results showed that the plantlet growth was favored in the 50 g/L or 70 g/L sucrose or white sugar,30/30 （mM/mM） NH4^＋/NO3^-, and 30 mM or 60 mM total nitrogen concentration. Considering the medium cost, the medium of MS＋ 50 g/L white sugar ＋ 30/30 （mM/mM）NH4^＋/NO3^- ＋ 30 mM total nitrogen was optimal for sweet potato growth in vitro. During the acclimatization stage, the survival ratecould reach 95 % when the plantlets were transferred in pots containing vermiculite and peat.