大鼠胸腺内猪胰岛异种移植的实验研究

Intrathymic islet transplantation: An experimental study

目的:观察胸腺内和肾包膜下胰岛移植对移植物存活时间的影响。方法:我们应用胰管内注射胶原酶的方法分离出猪胰岛为供体,以W istar糖尿病大鼠为受体,通过移植物的存活期来观察大鼠胸腺内及肾包膜下猪胰岛异种移植的效果。结果:单纯胸腺内移植的存活期(6.0±3.6)d,长于单纯肾包膜下组的(5.3±1.8)d。移植的同时加用抗大鼠胸腺淋巴血清(ATS),胸腺内移植+ATS的存活期为(21.7±9.1)d,明显长于肾包膜下移植+ATS组的(13.7±3.2)d。结论:胸腺可能为胰岛移植的理想部位,而且在诱导免疫耐受中具有重要作用。

Objective： To observe the effects of intrathymic islet transplantation or renal subcapsule islet transplantation on the graft survival, nethods：Wistar rats were used as recipients and pigs as donors. The recipients receiving intrathymic or renal subcapsule islet transplantation were divided into simple transplantation groups and the group intraperitoneally receiving rabbit antithymoeyte serum at the time of islet transplantation. Results：The median survival time （MST） of simple intrathymic xenografts was 6. 0 days, longer than that of xenografts implanted beneath the renal capsule. Administration of ATS at the time of islet transplantation delayed rejection of islets transplanted to the renal subcapsule （MST, 13. 7 days）,and intrathymic xenografts were superior. （MST, 21.7 days）. Conclusion：The thymus may be an ideal site for islet transplantation and may play an important role in the induction of immune tolerance.