摘要
目的:检测肾母细胞瘤(nephrohlastoma,wilms tumor,WT)组织中PTEN和MMP_7中的表达。方法:用免疫组化方法检测12例瘤旁正常肾组织和42例WT组织中PTEN、MMP_7的表达。结果:PTEN在WT组织中的表达明显低于正常瘤旁肾组织(P<0.05)。PTEN的表达与性别、年龄和病理学分型无关(P>0.05),而与临床分期明显相关(P<0.05)。12例对照组织标本中MMP_7未见表达,42例WT组织标本中MMP_7在胞浆表达率54.21%。MMP_7在预后良好(favorable histological types,FH)组中的阳性表达率明显低于在预后不良(unfavorable histological types,UH)组中的阳性表达率(P<0.05);PTEN和MMP_7呈负相关。结论:联合检测WT组织中PTEN和MMP_7的表达,能更好评估WT的侵袭和转移等生物学行为,两者可作为判断WT侵袭性的标志物。
To detect the expression of PTEN and MMP-7 in nephroblastoma. Methods： With immunohistochemistry to detect the expression of PTEN and MMP-7 in 12 cases of normal renal tissues near the tumor and 42 cases of children nephroblastoma tissues. Results ： Expression of PTEN was lower in nephroblastoma than that in normal renal tissues（ P 〈 0.05）. Expression of PTEN has no significant relativity with sex, age and pathologic types（ P 〉 0.05）. But significant relativity was found between PTEN and clinical stage（ P 〈 0.05）. There is no MMP-7 positive expression in 12 cases normal renal tissues, and 54.21% positive rate in 42 cases nephroblastoma tissues. There is a significant higher expression of MMP-7 in unfavorable histologic types nephroblastoma group than that of in favorable histologic types nephroblastoma group. A negative relativity was found between PTEN and MMP-7 in nephroblastoma tissues. Conclusion ： United detecting of PTEN and MMP-7 in nephroblastoma tissues, maybe stance helpful to estimate the biological behavior of nephroblastoma, and they can be the signal substance of estimating nephroblastoma＇ s metastasis.
出处
《河南医学研究》
CAS
2006年第2期121-123,共3页
Henan Medical Research
