高渗对豚鼠外毛细胞线粒体电位的影响

Hypertonic environment can affect the isolated guinea pig Outer hair cell membrane potenti

目的观察离体豚鼠耳蜗外毛细胞线粒体膜电位在高渗环境中的变化。方法解剖显微镜下仔细分离出第2—4回基底膜,采取外毛细胞,用粘附式细胞仪和线粒体膜电位特异性荧光探针观察外毛细胞(OHC)线粒体膜电位的变化。结果高渗环境可导致OHC线粒体内荧光强度降低,OHC形态发生变化。结论高渗透压可破坏OHC线粒体跨膜电位,进而影响其功能。

Objective To observe the variation of the isolated guinea pig Outer hair eell（OHC） membrane potential in the hypertonie solution. Methods To isolate the 2-4 basilannembranes under an anatomical microscope to investigate the potential changes in the OHC membrane by use of Adherent Cell Analysis and sorting eytometer （ACAS 570） and the specific fluorescent probe. Results Hypertonie environment can result in decreased fluorescent strength in OHC mitoehrondria and OHC morphologyical variation. Conclusions Hypertonie environment can impair the mitoehrondria transmembrane potential and furthermore affect it＇s function.