HPLC法测定壮骨关节丸中淫羊藿苷含量

HPLC method of controlling quality for Icariin in Zhuanggu Guanjie Pill

目的:建立高效液相色谱法(HPLC)测定壮骨关节丸中淫羊藿苷含量的方法。方法:采用HPLC法测定壮骨关节丸中淫羊藿苷的含量,以C18柱作为色谱柱,甲醇-1%冰醋酸(60:40)为流动相,检测波长为270 nm,按外标表法定量。结果:壮骨关节丸中淫羊藿苷的含量为0.498 7 mg/g。加样平均回收率为100.02%,RSD=1.58%(n=5);重现性试验的RSD=1.65%(n=6)。结论:为壮骨关节丸中药物的定性与定量分析提供了准确、灵敏的方法。

Objective： To study the method of controlling quality for Zhuanggu Guanjie Pill. Methods： Icariin in Zhuanggu Guanjie Pill was determined by HPLC, mobile phase was methanol-1% glacial acetic acid （60：40）, detective wave length was 270 nm. Results： The icariin in Zhuanggu Guanjie Pill was 0.4987mg/g.The mean recovery was 100.02%, RSD=1.58% （n=5）.RSD as repeatability index was 1.65% （n=6）. Conclusion： The methods are available with a good reproducibility and can be used to control the quality of Zhuanggu Guanjie Pill.