巨细胞病毒IgM抗体检验中消除RF干扰因素的实验研究

Research for eliminating interference of rheumatoid factor in detecting of human cytomegalovirus IgM

目的:为了提高ELISA法检测巨细胞病毒(HCMV)的敏感性。方法:用含聚合IgG的血清稀释液将血清标本稀释,检测30份类风湿因子(RF)阳性标本以及300份待检HCMV标本。结果:用含聚合IgG20μg/ml的血清稀释液稀释血清标本,去除血清中可能存在的RF,可预防试验出现假阳性反应结果。结论:在血清稀释液中加入聚合lgG20μg/ml,既保证试验敏感性又有效提高了酶联免疫吸附试验抗体捕获法检测IgM的特异性。

Objective： To increase sensitivity of ELISA for detecting human cytomegalovirus（HCMV）. Methods： We diluted sample with serum including ploy IgG.Then,we detected HCMV in 30 samples with rheumatoid factor （RF） and 300 samples without RF. Results： The samples diluted with serum including ploy IgG （20μg/ml）didn＇t reduce sensitivity in increases specificity and sensitivity of ELISA kits. ELISA kit. Conclusion： Adding poly IgG to samples increases specificity and sensitivity of ELISA kits.