摘要
目的:改进盐酸左氧氟沙星的含量测定方法。方法:采用C18反相柱,手性流动相为0.1%硫酸铜-0.15%L-苯丙氨酸溶液-甲醇(80∶20),流速为1.0ml·min-1,检测波长为293nm,柱温40℃。结果:按左氧氟沙星、右氧氟沙星的顺序流出,其分离度大于1.5;左氧氟沙星在0.41～6.1μg范围内线性关系良好(r=0.9999),平均回收率99.1%,RSD为0.09%(n=9)。结论:本法准确、可靠,适于左氧氟沙星的定性和定量。
Objective：To improve the determination of Levofloxacin Hydrochloride. Methods：Using C18 column,Chiral mobile phases consisted of the solution of 0. 1% CuSO4 ＆ 0. 15% L-phe-methanol（80 ： 20）. The flow rate was 1.0ml·min^-1 and UV detector at 293 nm. The temperature of column was 40℃. Results ：Eluting at Levofloxacin and dextrofloxacin in turn,the resolution was larger than 1.5. There was a good linearity in the range of 0.41～6. 1μg of Levofloxacin（r= 0. 9999）,and the mean recovery was 99. 1% with RSD 0.09% （n= 9）. Conclusion：The method is accurate and suitable for the qualititive and quantitative analysis of Levofloxacin.
出处
《中国药品标准》
CAS
2006年第3期29-31,共3页
Drug Standards of China
关键词
盐酸左氧氟沙星
手性流动相
Levofloxacin Hydrochloride
Chiral mobile phases
