HPLC法测定金蒲胶囊中苦参碱的含量

Content Determination of Matrine in Jinpu Capsules by HPLC

目的:建立高效液相色谱法测定金蒲胶囊中苦参碱的含量。方法:采用ZorbaxC18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-乙腈-磷酸盐缓冲液(PH6.8)-三乙胺(18∶18∶70∶0.1),流速1.0mL·min-1,检测波长220nm。结果:进样量在0.2116μg～1.2696μg范围内呈良好线性关系,其回归方程为Y=1105738.7X-4017.1(r=0.9999)。平均回收率98.6%(n=6),RSD=2.4%。结论:该法简便、灵敏、准确,重现性好,可用作含量测定。

Objective： An HPLC method was established for determination of Matrine in Jinpu Capsules. Methods ： The Zorbax SB-C18 column （4.6mm×250mm,5μm）was used. The mobile phase was consisted of methanol-acetonitrile-phosphate salt buffer solution（pH6.8）-triethylamine（18 ： 18 ： 70 ： 0. 1）,the flow rate was 1.0mL · min ^-1,the detection wavelengh was at 220nm. Results ：There was good linear within the range of 0. 2116～ 1. 2696μg,and regression equation was Y= 1105738. 7X- 4017.1 （r = 0.9999）. The average recovery was 98.6 % （n = 6）, RSD = 2. 4 %. Conclusion ： This method was convenient, sensitive,accurate and reproducible,which can be used for quality control.