重症监护病房感染阴沟肠杆菌的耐药性及基因型分析

Drug Resistance and Genotyping of Enterobacter Cloacae Infected in Intensive Care Units

目的了解我院重症监护病房(ICU)医院感染阴沟肠杆菌的耐药谱及分子流行病学特征,指导临床合理用药。方法基因分型采用优化反应体系的随机扩增多态性DNA(RAPD)法,耐药分析选用K-B法。结果RAPD分型将28株阴沟肠杆菌分为11种株型,耐药谱则将其分为12种不同的耐药型。结论基因分型和耐药分析显示ICU有多重耐药的阴沟肠杆菌株爆发流行现象,它为控制阴沟肠杆菌的医院内感染、追踪传染原、切断传播途径提供遗传学信息,指导临床医生选用敏感有效的抗生素。

Objective To study the type of resistance spectrum and molecular biological characteristics of Enterobacter cloacae infected in intensive care units. Methods Random amplified polymorphic DNA（RAPD）analysis was used for gene typing. K-B method was used for the determination of antibiotic resistance pattern. Results Twenty-eight clinical strains of E. cloacae were divided by RAPD into 10 types. And according to the antibiotic resistance patterns, the 28 strains were typed into 12 kinds. Conclusion Genotyping and analysis of resistance to antibiotics demonstrate the outbreak of multidrug resistant strains E. cloacae in intensive care units. It can not only provide genetic information for controlling, preventing hospital infection and tracing the infection source and the way of dissemination but also direct the clinical doctors to select sensitive antibiotics.