胃肠道恶性肿瘤Cyclins表达分型与MDR1及TNM分期的关系

Correlations of Cyclins expression types with multi-drug resistance-1 and TNM staging in gastrointestinal cancer

目的:研究胃肠道恶性肿瘤的4种主要Cyclins (Cyclin D1,E,A,B1)的表达规律,以此为依据对恶性肿瘤进行分型,并结合肿瘤标本中 MDR1(多药耐药基因1)的表达情况,与肿瘤 TNM分期进行相关性分析．方法:采用流式细胞术分析新鲜手术获取的 62例肿瘤标本(胃癌32例,结直肠癌30例)的 Cyclin D1,E,A,B1及MDR1表达情况,再记录术后病理报告进行TNM分期．结果:根据4种主要Cyclins的表达情况对恶性消化道肿瘤分为Ⅰ-Ⅳ型Cyclin非时相性表达类型,此Ⅰ-Ⅳ型细胞周期类型与TNM分期具有一致性,Kappa系数等于0．599(P<0．01)．Ⅰ- Ⅳ型细胞周期类型中MDR1的表达水平逐渐增高,等级相关系数rs为0．495(P<0．01)．结论:根据Cyclins的表达情况对消化道恶性肿瘤进行的分型具有与TNM分期相同的意义．在消化道肿瘤中,MDR1与肿瘤细胞周期素表达类型有关,对治疗具有指导意义．

AIM： To investigate the expression of four Cyclins in gastrointestinal cancers, and to explore their correlations with multi-drug resistance-1 （MDR1） and TNM staging. METHODS： Sixty-two fresh samples （gastric cancer 32, colorectal cancer 30） from patients underwent operation were collected, and the expression of Cyclin （D1, E, A, B1） and MDR1 were detected by flow cytometry. In addition, pathological reports were analyzed for TNM staging. RESULTS： According to the expression of four Cyclins, four cell cycle types were divided in the gastrointestinal cancers. These four cell cycle types were in accordance with TNM staging （κ = 0.599, P 〈 0.01） and marked correlation with the level of MDR1 expression （rs = 0.495, P 〈 0.01）. CONCLUSION： Cell cycle typing based on Cyclins expression has the same significance as TNM staging. MDR1 expression is correlated with the cell cycle typing, so it is possible to reverse MDR through interfering in cell cycle.