GCLM C-588T多态与中国人冠状动脉粥样硬化性心脏病遗传易感性的关系

Association of GCLM C-588T Polymorphism with Genetic Susceptibility to Coronary Heart Disease in Chinese Population

目的 探讨谷氨酸-半胱氨酸连接酶修饰亚单位基因（glutarnat-cysteine ligase modifier subunit gene，GCLM）C-588T多态与中国人冠状动脉粥样硬化性心脏病（coronary heart disease，CHD）遗传易感性的关系。方法 采用聚合酶链反应-限制性片段长度多态方法，检测209例CHD与240名对照的GCLM基因型分布及差异。结果 CHD组及对照组T等位基因频率分别为17．2％及26．3％，T的CHD发病风险是C的0．59倍（95％CI=0．42-0．81。P=0．001）。与C/C纯合子相比，C／T杂合子引起CHD风险为0.67倍（95％CI=0．45～1．01，P=0．056），而T／T纯合子引起CHD风险度显著降低至0.20倍（95％CI=0．06-0．61，P=0．005）。结论 GCLM-588T降低CHD发病风险，提示T/T基因型是中国人CHD的一个遗传保护因素。

Objective To investigate the possible association of the glutamate-cysteine ligase modifier subunit gene （GCLM） C-588T polymorphism with coronary heart disease （CHD） in a Chinese population. Methods For 209 CHD eases and 240 unrelated health individuals, GCLM C-588T genotypes were determined using a PCR-based restriction fragment length polymorphism （RFLP） method. Odds ratios （ORs） for HCC and 95 % confidence intervals （CIs） from unconditional logistic regression models were used to evaluate relative risks. Results The frequency of T allele was 0. 172 in CHD eases and 0. 263 in controls, respectively. The T allele had a significantly decreased risk for CHD （OR = 0.59, 95% CI= 0.42 - 0.81, P = 0. 001） in comparison with the C allele. Compared to C/C homozygote, C/T heterozygote had a 0.67-fold decreased risk （95 % CI = 0.45 - 1.01, P = 0. 056）, whereas T/T homozygote had a 0.20-fold signficantly decreased risk （95% CI=0.06-0.61, P=0.005） of CHD.Conelusion TheT alleleof GCLMC-588Tis associated with the decreased risk of CHD and T/T homozygote is potentially one of the protective factors for CHD in Chinese population.