瘦素及其受体在子宫内膜异位症中的表达

Leptin and Leptin Receptor Expression in Endometriosis

目的测定瘦素(leptin)及其受体在子宫内膜异位症组织中的表达,探讨瘦素及其受体与子宫内膜异位症的相关性。方法采用逆转录聚合酶链反应(RT-PCR)技术,检测21例手术后病理确诊的子宫内膜异位症患者的异位内膜及其诊刮的子宫内膜组织中瘦素受体mRNA表达,同时以17例因功能性子宫出血而行全子宫切除手术的子宫内膜组织中瘦素受体mRNA表达水平为对照。并采用放射免疫法(RIA)检测两组患者血清中瘦素水平。结果子宫内膜异位症患者与对照组血清瘦素水平比较差异无显著性(P>0.05)。异位子宫内膜组与自体子宫内膜及对照子宫内膜均有瘦素受体表达,而前者与后两者之间有显著性差异(P<0.05),后两者之间无显著性差异(P>0.05)。结论瘦素及其受体并非通过影响体内激素代谢而导致子宫内膜异位症的发生,可能参与腹腔内环境的改变而成为子宫内膜异位症发生的相关因素之一。

Objective To investigate the relativity of leptin and leptin receptor to endometriosis by detecting their expression in endometriotic tissue. Methods Leptin receptor mRNA concentration of endometriotic tissue and endometrium of 21 women with endometriosis diagnosed by pathology was detected by reverse transcription-polymerase chain reaction （RT-PCR）. Seventeen controls were hysterectomy women with functional uterine bleeding. Radioimmunoassay （RIA） was used to examine serum leptin levels of two groups. Results No significant difference in the serum leptin levels was found between the studied groups （P 〉 0.05）. Leptin receptor was expressed in both endometriotic tissue and endometriurn, both experimental and control group. Significant difference was observed between endometriotic tissue and endometrium, including control group （P 〈 0.05）, while no significant difference existed in the endometrium of two groups （P〉0.05）. Conclusion Leptin and lepfin receptor can not lead to endometriosis by affecting hormone metabolism in vivo. Maybe they are related factors in the pathogenesis of endometriosis as involved in the change of intraabdominal environment.