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V806M突变型Axin基因真核表达载体的构建及其在神经胶质细胞瘤内的表达 被引量：1

Construction of the eukaryotic expression vector of V806M mutant Axin and its expression in glioma cells
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摘要 目的构建V806M突变型Axin基因真核表达载体pIRES2-EGFP-MT-Axin(V806M),并稳定表达于大鼠神经胶质瘤细胞系C6。方法用分子克隆技术,构建真核表达载体pIRES2-EGFP-MT-Axin(V806M),经NheⅠ和SmaⅠ双酶切鉴定后,用脂质体法稳定转染神经胶质瘤细胞C6。结果构建了真核表达载体pIRES2-EGFP-MT-Axin(V806M),稳定转染后,经荧光显微镜和免疫细胞化学染色法检测,可见细胞内有EGFP及Axin的表达。结论成功构建真核表达载体pIRES2-EGFP-MT-Axin(V806M),并在神经胶质瘤细胞C6中表达,为研究此种突变体Axin是否影响细胞的生物学行为以及是否参与胶质瘤的发生发展奠定了实验基础。 Objective： To construct the eukaryotic expression vector plRES2 - EGFP - MT - Axin （ V806M ）, and to express mutant Axin in C6 glioma cells. Methods： The eukaryotic expression vector plRES2 - EGFP - Axin was constructed by introducing mutant Axin（V806M） DNA fragment into the sites of Nhe Ⅰ and Sma Ⅰ of plRES2 - EGFP vector. The plasmid was transfected into the C6 cells using hpofectamine. Results： The eukaryotic expression vector plRES2 -EGFP - MT -Axin（V806M） was constructed and transfected successfully into C6 glioma cells. The green fluorescence of EGFP was observed in the plasma and nuclei of transfected cells, and Axin protein was only found in the plasma. Conclusion. The recombinant expression vector plRES2 - EGFP - MT - Axin （ V806M ） was constructed, and the EGFP and Axin gene could be co - expressed in the C6 cells. This study laid a foundation for the further research of the function of mutant Axin in cell growth and tumorigenesis.
作者 李烦繁 李青 陈广生 张丽英 洪柳 王淑芳 姚丽
机构地区 第四军医大学西京医院病理科
出处 《现代肿瘤医学》 CAS 2006年第7期800-802,共3页 Journal of Modern Oncology
基金 国家杰出青年自然科学基金项目(No.30125012) 军队医药卫生科研基金项目(No.02ma04)
关键词 突变型Axin EGFP 真核表达载体 神经胶质瘤细胞C6 mutant Axin EGFP eukaryotic expression vector C6 glioma cell
分类号 R730.3 [医药卫生—肿瘤]
  • 相关文献

参考文献11

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二级参考文献10

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共引文献6

同被引文献15

  • 1张丽英,李青,陈广生,叶菁,张丰,李繁烦,林圣彩.Axin基因的表达对神经胶质瘤细胞系C6生物学特性的影响[J].现代肿瘤医学,2006,14(5):521-524. 被引量：1
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现代肿瘤医学
2006年 第7期

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