酪丝缬肽对裸小鼠人肝癌BEL-7402移植瘤的抑制作用

Tyroservatide inhibits the growth of human hepatocarcinoma in nude mice

目的观察酪丝缬肽对裸小鼠人肝癌BEL-7402移植瘤的抑瘤作用,并初步探讨其可能的作用机制。方法通过动物模型观察酪丝缬肽对裸小鼠人肝癌BEL-7402移植瘤肿瘤重量及体积的影响；以电镜观察肿瘤细胞超微结构的改变；以免疫组化法观察瘤组织中增殖细胞核抗原(PCNA)的表达；以利用TUNEL法检测瘤组织细胞的凋亡情况。结果给药剂量为80μg·kg^(-1)·d^(-1)和160μg·kg^(-1)·d^(-1)时,酪丝缬肽可以显著抑制裸小鼠BEL-7402移植瘤的生长,抑瘤率分别为64．02%和66．27%,与生理盐水及相应氨基酸混合物组比较,差异均有统计学意义(P＜0．05)。酪丝缬肽可引起肿瘤细胞超微结构改变,导致肿瘤细胞广泛坏死和凋亡。酪丝缬肽给药组与生理盐水组比较,肿瘤组织细胞的PCNA表达明显降低,凋亡细胞明显增多。结论酪丝缬肽可显著抑制人肝癌BEL-7402细胞在裸小鼠体内的生长,抑制肿瘤细胞增殖,促进瘤细胞凋亡。

Objective To investigate the inhibitory effects of tyroservatide and its amino acid mixture on growth of hepatocarcinoma. Methods Hepatocarcinoma in nude mice was induced by implantation of cells of human hepatocarcinoma cell line BEL-7402, The inhibition of hepatocarcinoma growth was determined by calculating the tumor volume and measuring the tumor weight, The effects of tyroservatide on tumor cells in nude mice were assessed by immunohistochemical staining of proliferating cell nuclear antigen （ PCNA ）, electron microscopic observation of uhrastructure, and apoptosis of tumor cells using terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling （TUNEL）. Results Tyroservatide significantly inhibited the growth of human hepatocarcinoma in nude mice, with an inhibiting rate more than 60%. But the mixture of amino acid did not show a significant inhibitory effect on the tumor growth. Tyroservatide also induced apoptosis of tumor cells and decreased the expression of PCNA in tumor cells, Conclusion Tyroservatide may significantly inhibit the growth of human hepatocarcinoma in nude mice by inducing apoptosis and inhibiting proliferation of tumor cells.