构建鼠IL－6反义RNA的逆转录病毒重组体

CONSTRUCTION OF RETROVIRAL VECTOR FOR EXPRESSIVE MOUSE INTERLEUKIN-6

用限制性内切酶消化、提取鼠白细胞介素６ｃＤＮＡ全基因，并克隆至逆转录病毒穿梭质粒ｐＺＩＰ－ｎｅｏＳＶ（ｘ）ＢａｍＨⅠ位点，经斑点杂交，限制性内切酶消化，电泳鉴定，证实成功地构建了表达鼠ＩＬ－６反义ＲＮＡ的逆转录病毒重组体，从而为研究白细胞介素６基因转移与治疗提供了物质基础。

The mouse IL-6 cDNA was isolated by restriction enzyme XhoⅠfrom plasmid TXM/ IL-6 cDNA and inserted into the BamH Ⅰsite of the retroviral vector pZIP to construct the recombinant plasmid pZIP IL-6.It was screened and identified by agrose gel electrophoresis, restriction enzyme Bgl Ⅱanalysis and nucleic acid hybridization, the results evidenced that pZIP IL-6 contained the correctly inserted IL-6 cDNA fragment. This study provides a fundament for using recombinant retroviru-mediated antisense IL-6 gene transfer and therapy.