HPLC测定冰茶栓中茶叶咖啡碱的研究

Determination of Caffeine in Bingcha Suppository for Treating Morphine Addicts by HPLC

应用HPLC高效液相色谱法测定戒毒制剂冰茶栓中茶叶咖啡碱的含量。以乙醚一石油醚（1：1）溶解栓剂，并用50％甲醇水溶液萃取，再经HPLC测定。测定条件为：ODS-C18分析色谱柱（150mm×4．6mm，5μm），流动相0．02mol·L^-1醋酸铵：甲醇（80：20），流速1mL·min^-1，检测波长270nm。结果显示其回归方程为Y=0．9370＋0．00002X（r=0．99999），线性范围为10～250μg·mL^-1，平均回收率、精密度、重复性、稳定性均符合要求，且不受基质等其它成分的干扰。HPLC方法用于测定冰茶栓中咖啡碱含量结果准确可行。

The Objective of this research was to research the quantitative method of tea caffeine in Bingcha Suppository for treating morphine addicts by using HPLC. The Suppository was solved by ether： petroleum ether （ 1 ： 1 ）, extracted by 50% methanol aqueous solution, then determined by HPLC. The conditions were ODS - C18 （ 150mm × 4.6mm, 5μm） colmnn, 0.02mol·L^-1 ammonium acetate： methanol （80：20） mobile phase, flow rate 1mL. min^-1, wavelength 270nm. The result showed the regression equation Y = 0.9370 ＋ 0.00002X （r= 0.99999）, the linear range 10 - 250μg·mL^-1, the average recovery, precision, repetition and stability were complied to the limit. The test results were not affected by the ground substance and other components in the suppository. Those showed this method was accurate and practicable.