唐松草的组织培养与快速繁殖技术

Tissue Cuture and Rapid Propagation of Thalictrum aquilegifolia

通过对唐松草的组织培养,研究了不同激素配比对愈伤组织、芽的诱导分化和增殖的影响以及驯化移栽时基质对于成活率的影响。结果表明:诱导愈伤组织的适宜培养基为MS+1.0 mg/L6-BA+0.5 mg/L2,4-D;诱导芽分化的适宜培养基为MS+2.0 mg/L6-BA+0.2 mg/LNAA;适宜继代培养基为MS+1.0 mg/L6-BA+0.2 mg/LNAA;适宜生根培养基为1/2 MS+0.5 mg/LNAA。同时还表明,炼苗时选用珍珠岩与蛭石以11∶的比例混合的基质的成活率最高。

In this article, the influence of several factors on micropropagation of Thalictrum aquilegifolia was investigated. The results showed that the appropriate media for the callus induction was MS ＋ 1.0 mg/L 6-BA ＋ 0.5 mg/L 2,4-D,buds differentiation and subculture medium was MS ＋ 2.0 mg/L 6-BA ＋ 0.2 mg/L NAA,MS ＋ 1.0 mg/L 6-BA ＋ 0.2mg/L NAA,respectively;the best medium for initiation and growth was 1/2 MS ＋ 0.5 mg/L NAA. The plantlets were transplanted to perlite and vermiculite（ 1：1 ） where the plant grew well,and the survival rate was 85 %.