大孔树脂纯化无梗五加果总黄酮工艺研究

Study on the Purification of Flavonoids from Fruit of Acanthopanax Sessiliflorus Seem with Macroporous Absorbing Resin

对大孔吸附树脂纯化分离无梗五加果总黄酮的工艺进行了研究。试验表明:HPD600树脂对无梗五加果总黄酮具有较好的吸附和解吸效果,最佳纯化工艺条件为:上样液pH=5.06,浓度为0.5mg/ml,结果与树脂用量比为101∶,控制流速为1ml/min,以64ml体积分数为70%的乙醇解吸,最终得到的黄酮纯度可达86.79%。

In this experiment,the macroperous absorbing resin is used to purify and separate flavonoids. The experimental results showed： HPD600 resin had better absorbing and conciliating ability. The pH and the concentration of the original solution was 5.06 and 0.5 mg/ml;the conciliating eluent was 70 % ethanol and the velocity of flow was 1 ml/min. In this condition, the purity of flavonoids was 86.79 % .