血清登革热病毒NEST-PCR检测

Detection of Dengue Virus From Patients's Serums by Nest-PCR

目的 建立用NEST—PCR快速检测登革热病毒的方法。方法 通用引物对样本进行RT—PCR扩增，然后用分型引物进行NEST—PCR，根据产物条带的位置分型。结果 标准毒株的扩增结果与预期结果相同，血清登革热病毒在病程2～5d的阳性率高于6～9d的阳性率，分别为6／7和2／6。结论 NEST—PCR方法是一个快速、准确检测登革热的方法，但它更适用于早期临床检测。

Objective To set up a rapid method for detection of dengue virus by NEST- PCR. Methods A set of random primers were used for RT - PC＇T, and then type specified primers were used for NEST - PCR. Types could be identified by the length of the product. Results The amplification result of standard strain were as same as the anticipated result. The positive rate of the early serums （85.7 96 ） was higher than that of the later ones （33.33 % ）. Conclusions The method is a rapid and exact way to detect dengue virus. And it seems more suitable for early clinical detection.