板蓝根、黄芪对猪繁殖与呼吸综合征病毒的体外抑制作用

In vitro inhibition of porcine reproductive and respiratory syndrome virus by Radix isatidis and Astragalus

为了解中草药黄芪、板蓝根对病毒增殖的抑制作用,对黄芪、板蓝根及二者联合使用在Marc-145的单层细胞上对猪繁殖与呼吸综合征病毒(PRRSV)增殖的抑制作用进行了研究。结果表明,板蓝根能够显著抑制PRRSV体外增殖,对PRRSV的最小直接杀灭浓度为0.195 mg/mL,最小阻断浓度为0.097 mg/mL;黄芪对PRRSV增殖的抑制作用较弱。板蓝根、黄芪联合使用时对PRRSV的抑制作用显著增强,板蓝根对PRRSV的最小直接杀灭浓度降为0.097 mg/mL,最小阻断浓度降为0.049 mg/mL。

The anti-PRRSV （porcine reproductive and respiratory syndrome virus） activities of Radix isatidis and Astragalus were studied by testing the inhibition to cytopathogenic effect （CPE） in Marc-145 ceils. The minmal inhibitory concentration （MIC） was determined directly and indirectly. The results showed that the anti-PRRSV activity of 1 ： 1 mixture of Radix isatidis and Astragalus was significantly higher than that of Radix isatidis or Astragalus alone.