白花曼陀罗细胞悬浮培养生物转化外源氢醌合成熊果苷的研究(英文)

Production of Arbutin through Biotransformation of Exogenous Hydroquinone by Datura stramonium Cell Suspension Cultures

研究了白花曼陀罗细胞悬浮培养对外源氢醌的糖基化。转化细胞来自白花曼陀罗嫩茎在LS固体培养基上诱导产生的愈伤组织。白花曼陀罗悬浮培养细胞不能分泌熊果苷,但能糖基化外源氢醌合成熊果苷。当氢醌添加量达240μmol/100 mL培养物时,约有93.4%的氢醌转化形成了熊果苷,并应用多种色谱技术进行分离纯化,进行了HPLC分析和结构鉴定。

To investigate the biotransformation of hydroquinone by cell suspension cultures of Datura stramonium. Cultured cells derived from stems of Datura stramonium were maintained in Linsmaiher and Skoog （LS） solid medium. Datura stramonium cells in suspension cultures did not accumulate aro butin （4-hydroxyphenyl-β-D-glucopyranoside） but were able to specifically o-glucosylate exogenous hydroquinone at position 1. In particular, Datura cultures glucosylated ca 93.4% of hydroquinone （240 μmol/100 mL cultures） within 8 days after hydroquinone administered. The arbutin obtained was extracted from the cultures and further purified by silicon Gel column chromatography. The exogenous hydroquinone and arbutin were analyzed by HPLC.