新型免疫增强蓝藻作为日本鳗鲡黑仔饲料添加剂的实验研究

Experimemtal Study on One New Immunostimulated Blue Algae as Additive in Feed for Anguilla japonica Black Fry

免疫增强蓝藻是把胸腺素α1（Tα1）基因转移到蓝藻-聚球藻PCC7942（Synechococcus sp．PCC7942）染色体上后获得稳定表达的转基因蓝藻，经小鼠实验初步表明其有生物学活性并且口服有效．本实验采用该免疫增强蓝藻饲喂日本鳗鲡（Anguilla japonica）黑仔，并采集分离了一种养殖鳗鱼流行性发病弧菌（Vibrio sp．），对饲喂了免疫增强蓝藻的黑仔鳗进行感染试验．实验表明饲喂添加免疫增强蓝藻的饲料后，黑仔鳗各检测组织器官Tα1含量显著提高，免疫增强蓝藻提高了试验鱼的免疫能力，增强了抗逆、抗病菌感染能力，并具有一定的促生长作用．免疫增强蓝藻作为鱼类饲料添加剂适口性好，适宜添加量为（0．5～1）×10^-6左右．

The immunostimulated blue algae is a effectively expressed transgenic blue algae which has translated human thymosinalpll（Tα1） gene to Synechococcus sp. PCC7942. It has been confirmed that immunocompetence is available to mice and can be utilized by oral administration. We fed japonica eel with the immunostimulated Synechococcus sp. as immunostimulated additive, isolated an epidemic pathogenic Vibrio sp. （04-09）from the diseased japonica eel, infected the black fry which were fed with the immunostimulated blue algae with Vibrio sp.. The experiment shows that contents of Tα1 in the tissues and organs of the black fry increased obviously. The immunostimulated Synechococcus sp. can elevate the immune ability of japonica eel, increase the ability of resisting the bacterial pathogens such as Vibrio sp. and environmental stresses, stimulate the growth of japonica eel. The immunostimulated blue algae is an appropriate additive of copulated feed for the rare aquicuhured fish and additive dosages is about （0. 5-1.0）×10^-6.