重症肌无力转基因小鼠模型的建立

Institution of model of myasthenia gravis in transgenic mice

[目的]建立人免疫球蛋白转基因小鼠实验性自身免疫性重症肌无力模型．[方法]用电鳐电器官分离纯化的乙酰胆碱受体免疫人免疫球蛋白转基因小鼠，以肌肉收缩力判定病情，以放射免疫测定法测定动物血清中抗乙酰胆碱受体抗体滴度及动物全身肌肉乙酰胆碱受体浓度．[结果]实验动物血清中抗乙酰胆碱受体抗体滴度为6～296nmol，全身肌肉乙酰胆碱受体损失率最高达65％，部分动物出现肌肉收缩无力表现．[结论]成功地建立了人免疫球蛋白转基因小鼠实验性自身免疫性重症肌无力模型．

OBJECTIVE To set up a new model of experimental autoimmune myasthenia gravis （EAMG） in mice transgenic for human immunoglobulin （Ig） loci. METHODS Mice transgenic for human Ig were immunized with acetylcholine receptor （AChR）, isolated and purified from electronic organs of Torpedo. The severity of clinical signs of disease was scored by measuring muscular weakness. The anti-Torpedo AChR antibody titer, and the concentration of AChR in muscle were determined by radioimmunoassay. RESULTS The titers of anti-Torpedo AChR antibodies in sera ranged from 6 to 296 nmol. The AChR loss in the muscle of Torpedo AChR-immunized mice was up to 65% compared to control mice. Furthermore, the muscular weakness was induced in some of the mice CONCLUSION A model of EAMG was successfully induced in mice transgenic for human Ig.