促红细胞生成素促进脑梗死后神经元再生

Treatment of ischemic stroke with erythropoietin enhances neurogenesis in SCID mice

目的观察促红细胞生成素(EPO)对脑梗死后神经元再生的作用。方法电凝并切断SCID小鼠左侧大脑中动脉,制作皮质脑梗死模型。24h后每天腹腔注射EPO,连续7d;对照组注射等量容积的PBS。每天腹腔注射bromodeoxyuidine(BrdU)用于标记分化细胞,连续10d。第35天取脑测定梗死边缘皮层神经元再生情况和皮层宽度变化。结果EPO治疗组皮质宽度指数为0·935±0·017,明显高于对照组的0·876±0·009(P<0·05)。在脑梗死边缘皮质中,EPO治疗组BrdU和NueN双标阳性的新生神经元为(16·8±4·3)HP,明显高于对照组的(3·5±1·5)HP(P<0·05)。结论EPO对于脑梗死后神经元的再生有促进作用。

Objective To investigate the role of erythropoietin（EPO） in neurogenesis and cortex expansion after permanent cerebral ischemic stroke Methods Animal model was induced in SCID mice Left middle cerebral arteries of SCID mice were occluded and cut by a bipolar electrocoagulator at the site 1-2 mm lateral middle cerebral artery from left olfactory tract under microscopy. EPO（in group T） or the same volume of BPS（in group C） was administrated intraperitoneally daily for 7days staring at 24 hours after stroke For labeling proliferation cells, bromodeoxyuidine（BrdU） was injected intraperitoneally daily for 10 days into mice staring at 24 hours after stroke All mice were euthanized on the 35 th day after stroke for the evaluation of neurogenesis and cortical expansion. Results Compared with group C, EPO significantly increased the numbers of anti-BrdU and anti-neuronal nuclei （NeuN） double positive cells at the edge of infarct [（3.5 ± 1.5）HP and （16. 8 ± 4. 3）HP, respectively] （P〈0. 05） and induced the cortical expansion after stroke [the cortex width indexes were （0. 876±0. 009） and （0. 935 ±0. 017）, respectively（P〈0. 05）]. Conclusion Our data suggest that administration of EPO provided therapeutic benefit after stroke involved in neurogenesis and cerebral cortex expanding.