摘要
背景与目的:研究PTEN和P53基因在原发性胆囊癌(HGC)中的表达,以探讨它们在HGC发生发展中的作用,寻找胆囊癌诊断及预后的指标。方法:应用原位杂交(DNA-RNA)技术与免疫组化SP法分别检测64例HGC中PTEN mRNA、PTEN蛋白和P53蛋白的表达。以22例胆囊腺瘤及10例慢性胆囊炎作为对照。结果:①PTEN mRNA和蛋白在HGC组织中的阳性率(56.3%,59.4%)均明显低于胆囊腺瘤组织(81.8%,90.8%)和慢性胆囊炎组织(100%,100%),(P<0.05)。②P53蛋白在HGC组织中的阳性率(62.2%)均明显高于胆囊腺瘤组织(27.2%)和慢性胆囊炎组织(0.0%),(P<0.05)。结论:PTEN缺失和P53突变型蛋白共同参与了HGC的发生发展。
Background and purpose: To study the expression of PTEN and P53 and analyse their roles in HGC oncogenesis , and to discover a good marker for diagnosis and prognosis of HGC. Methods: The expression of PTEN mRNA, PTEN protein and P53 protein were detected by in situ hybridization(DNA-RNA) and immunohistochemistry( SP method) in 64 cases of HGC. We took 22 cases of gallbladder adenoma and 10 cases of chronic cholecystitis as controls. Results: (1) The positive expression rates of PTEN mRNA and protein in HGC( 56.3%, 59.4% ) were significantly lower than that in gallbladder adenoma (81.8%, 90.8% ) and chronic cholecystitis( 100%, 100% ) ( P 〈 0.05). (2) The positive expression rates of P53 protein in HGC(62.2%) were significantly higher than those in gallbladder adenoma (27.2%) and chronic cholecystitis (0.0%) (P 〈0.05). Conclusions: PTEN and, P53 may play important roles in HGC oncogenesis.
出处
《中国癌症杂志》
CAS
CSCD
2006年第7期547-550,共4页
China Oncology
关键词
原发性胆囊癌
P53
PTEN
免疫组化
原位杂交
primary gallbladder carcinoma
P53
PTEN
immunochemistry
in situ hybridization