原发性胆囊癌中PTEN和P53基因表达的研究

Study on the expression of PTEN and P53 in human primary gallbladder carcinoma(HGC)

背景与目的:研究PTEN和P53基因在原发性胆囊癌(HGC)中的表达,以探讨它们在HGC发生发展中的作用,寻找胆囊癌诊断及预后的指标。方法:应用原位杂交(DNA-RNA)技术与免疫组化SP法分别检测64例HGC中PTEN mRNA、PTEN蛋白和P53蛋白的表达。以22例胆囊腺瘤及10例慢性胆囊炎作为对照。结果:①PTEN mRNA和蛋白在HGC组织中的阳性率(56.3%,59.4%)均明显低于胆囊腺瘤组织(81.8%,90.8%)和慢性胆囊炎组织(100%,100%),(P<0.05)。②P53蛋白在HGC组织中的阳性率(62.2%)均明显高于胆囊腺瘤组织(27.2%)和慢性胆囊炎组织(0.0%),(P<0.05)。结论:PTEN缺失和P53突变型蛋白共同参与了HGC的发生发展。

Background and purpose： To study the expression of PTEN and P53 and analyse their roles in HGC oncogenesis , and to discover a good marker for diagnosis and prognosis of HGC. Methods： The expression of PTEN mRNA, PTEN protein and P53 protein were detected by in situ hybridization（DNA-RNA） and immunohistochemistry（ SP method） in 64 cases of HGC. We took 22 cases of gallbladder adenoma and 10 cases of chronic cholecystitis as controls. Results： （1） The positive expression rates of PTEN mRNA and protein in HGC（ 56.3%, 59.4% ） were significantly lower than that in gallbladder adenoma （81.8%, 90.8% ） and chronic cholecystitis（ 100%, 100% ） （ P 〈 0.05）. （2） The positive expression rates of P53 protein in HGC（62.2%） were significantly higher than those in gallbladder adenoma （27.2%） and chronic cholecystitis （0.0%） （P 〈0.05）. Conclusions： PTEN and, P53 may play important roles in HGC oncogenesis.