唑来膦酸对肺癌细胞生长抑制的观察及机制的初步研究

Study on the mechanism of zoledronic acid inhibiting growth and inducing apoptosis of lung cancer cell line A-549

目的：研究唑来膦酸对非小细胞肺癌（NSCLC）细胞株A-549生长抑制及诱导凋亡的作用。方法：用MTT法测定唑来膦酸、泰索帝及二药联合对肺癌细胞A-549的生长抑制率，流式细胞仪检测细胞周期、细胞凋亡率。结果：0．1—100μmol／L的唑来膦酸对肺癌细胞A-549均有抑制作用，首先表现为G1期阻滞，继而出现细胞凋亡，呈剂量、时间依赖性，并且与泰索帝联用可以提高细胞凋亡率。结论：唑来膦酸对肺癌细胞A-549具有明显抑制作用，且与泰索帝联用有协同作用，其作用机制可能是通过诱导细胞凋亡来实现的。

Objective：To study the effects of growth inhibition and apoptosis induced by zoledronic acid in NSCLC cell line A- 549. Methods：Investigate the effect of zoledronic acid,taxotere and the combination of both on proliferation of NSCLC cell line A-549 by MrlT assay. Flow cytometry was used to examine cell cycle, cell apoptosis rate. Resuits：Zoledronic acid could inhibit the proliferation of lung cancer cells in vitro,which was associated with arresting of Gl phase and inducing apoptosis by a time-dependent and dosedependent manner. The apoptosis rate of cell increased after zoledronic acid combined with taxotere. Conclusion：Zoledronic acid can induce apoptosis and block cell cycle of lung cancer cells. Zoledronic acid has also shown synergistic antitumor effects when combined with taxotere.