HBV慢性感染与CXC趋化因子表达相关性研究

STUDY ON THE RELATIONSHIP BETWEEN CHRONIC INFECTION OF HBV AND EXPRESSION OF CXC CHEMOKINES

目的：探讨HBV慢性感染与CXC趋化因子mRNA表达水平的相互关系。方法：以Real-time PCR法动态检测慢性乙肝患者IFN-α联合利巴韦林治疗前、治疗3个月、治疗6个月后IP-10、Mig mRNA的表达水平。以趋化因子与GAPDH比值为其最终含量。结果：慢性乙肝患者HBV-DNA平均水平6．07×10^5 copies／ml，ALT平均水平234.6U/L，IP-10、Mig mRNA表达水平分别为（0．7387±0．0768）、（0．6883±0．0693），与正常对照相比，差异有统计学意义（P〈0．001）。IFN-α治疗3个月后HBV-DNA平均水平1.30×10^4 copies／ml，IP-10、Mig mRNA表达水平分别为（0．5741±0.0912）、（0．5962±0．0987），与治疗前相比，差异有统计学意义（P〈0．001-P〈0.05）。IFN-α治疗6个月后HBV-DNA平均水平3．42×10^3 copies／ml，IP-10、Mig mRNA表达水平分别为（0．4235±0．0924）、（0．4105±0.0898），与治疗前相比，差异有统计学意义（P〈0．001）。IFN-α治疗后，ALT水平正常组IP-10、Mig mRNA也接近正常水平，与同期治疗ALT升高组相比，差异有统计学意义（P〈0．001）。结论：慢性乙肝患者IP-10、Mig mRNA表达升高，与体内HBV-DNA复制水平、血清ALT水平有关。IFN-α能显著抑制HBV复制，间接下调IP-10、Mig mRNA表达水平，IFN这种综合作用效果，对降低患者体内炎性反应、控制病情发展十分有利。

Objective： To study the relationship between chronic infection of HBV and expression of CXC chemokines rnRNA. Methods： The different levels of IP- 10 and Mig mRNA in peripheral blood of chronic hepatitis B were kineticaUy detected by real - time quantitative polymerase chain reaction （Real- time PCR） in different treatment stages of interferon-α （IFN - α）. The rate of chemokine/GAPDH was regarded as the extreme level of chemokine. Results： The mean levels of HBV- DNA, alanine aminotransferase （ALT） and expressive levels of IP- 10, Mig mRNA in peripheral blood of patients were 6.07 ×10^5 oopies/ml, 234.6 U/L, （0.7387 ± 0.0768）, （0.6883 ±0.0693）, respectively. It was significantly different in patients with chronic hepatitis B than that in normal oontrols （P〈0.001）. After treatment for three months, the mean level of HBV-DNA and the expressive levlesof IP-10, Mig mRNA were 1.30× 10^4 oopies/ml, （0.4235 ± 0.0924）, （0.4105 ± 0.0898）, respectively. These were significantly higher than that in normal oontrols （P〈0.001）. The expression levels of IP-10 and Mig mRNA in patients with normal ALT were also adjacent to normal level after treatment for six months. There was significant difference in subgroup with high level ALT compared with that in subgroup with mormal level ALT （P〈0.001）. Conclusion： This study shows that the expression of IP-10 and Mig mRNA is high in patients with chronic hepatitis B which is associated with the level of replication of HBV and the level of ALT. IFN-α can effectively restrain the replication of HBV and down - regulate the levels of IP- 10 and Mig mRNA to adjacent normal level in patients with chronic hepatitis B. IFN -α plays an important role in down - regulating inflammation response and oontrolling the development of the patients＇ condition.