褪黑激素对大鼠缺血再灌注纹状体降钙素基因相关肽和内皮素表达的影响

Effects of melatonin on CGRP and ET-1 in the striatum after the global ischemia and reperfusion of rat

目的:通过检测大鼠全脑缺血后再灌注纹状体内皮素(ET)、降钙素基因相关肽(CGRP)的表达和丙二醛(MDA)的含量变化,探讨ET和CGRP与脑缺血再灌注后神经元损伤的关系及褪黑激素(MT)对缺血再灌注纹状体ET、CGRP表达的影响。方法:于大鼠“四动脉结扎法”全脑缺血(30 m in)再灌注后第0、1、2、6 h分别腹腔注射褪黑激素2.5 mg/kg和10 mg/kg两个剂量,各组大鼠再灌注24 h后测定MDA含量,并用放射免疫法检测纹状体ET和CGRP含量变化。结果:MT 2.5 mg/kg和10 mg/kg两个剂量于大鼠全脑缺血(30 m in)再灌注后第0、1、2、6 h分别腹腔注射可降低再灌注24 h大鼠纹状体ET的表达,加强CGRP的表达,并降低了MDA的含量。结论:MT可能通过影响再灌注过程中ET和CGRP的表达,降低缺血神经元脂质过氧化反应,从而改善缺血再灌注神经元的延迟性损伤。

Objective： By detecting the change of ET-1, CGRP and MDA concentrations in the striatum of rat after the global ischemia and reperfusion, to study mechanism and the protective role of MT for global ischemia and reperfusion injury. Methods： MT was injected intraperitoneally at 0 h, 1 h, 2 h, 6 h after ischemia（30 min） induced by the ＂occlusion of four arteries＂, 2.5 mg/kg or 10 mg/kg each time, respectively. Researching the changes of ET and CGRP with radioimmunoassey; the changes of MDA concentration 24 hour after reperfusion. Results： At these does, MT could decrease ET expression but increase CGRP expression, decrease the content of MDA at 24 h after global ischemia （30 min）/reperfusion. Con- clusion： MT could improve delayed injury of neuron after CGRP, decrease the degree of lipids-peroxid, as well as. reperfusion with influence expression of ET and