内毒素诱导肾小球系膜细胞白细胞介素─6的产生及基因表达

EndotoxininducedIL6productionandgeneexpresioninratglomerularmesangialcels

目的：观察体外培养的大鼠肾小球系膜细胞在内毒素（ＬＰＳ）诱导下ＩＬ┐６的产生及其ｍＲＮＡ表达。方法：按经典方法培养肾小球系膜细胞（ＭＣ）、转种培养后分为二组，诱导组加ＬＰＳ１０ｍｇ／Ｌ，对照组不加，用分子生物学和免疫学方法进行检测。结果：ＩＬ┐６在正常ＭＣ中分泌量极微，其ｍＲＮＡ也为低水平表达，经ＬＰＳ诱导后二者均明显增加。ＩＬ┐６蛋白的产生６～１２ｈ达高峰，比对照组增加３～５倍，ＩＬ┐６ｍＲＮＡ的表达３～６ｈ达高峰，较对照组增加２～３倍。结论：ＬＰＳ可诱发系膜细胞ＩＬ┐６水平增高，可能作为炎症介质参与肾脏疾病的发病。

nthisstudy,IL-6productionandgeneexpressionwereinvestigatedinculturedmesangialcelswithorwithoutthestimulationofLPS.METHODSIL-6productioninculturedSDratmesangialcels(MCs)wasmeasuredbyELISA,andIL-6mRNAexpressiondetectedbyNorthernblotassay.ComparisonwasmadebetweennormalmesangialcelsandLPSco-culturedmesangialcels.RESULTSTheproductionandmRNAexpressionofIL-6inMCsstimulatedbyLPSweresignificantlyin-creasedascomparedwithnormalcontrol.TheproteinproductionofIL-6reachedpeakat6～12hours,whichwas3～5timeshigherinLPSstimulatedMCsthaninnormalMCs.PeaklevelofIL-6mRNAexpressionap-pearedat3～6hoursandwas2～3timeshigherinLPSstimulatedMCsthaninnormalMCs.CONCLUSIONLPSpotentiatestheefectofIL-6ontheproliferationofMCsinculture.IL-6playsaroleinthepathogenesisofdevelopmentofMsPGN.