摘要
目的:观察体外培养的大鼠肾小球系膜细胞在内毒素(LPS)诱导下IL┐6的产生及其mRNA表达。方法:按经典方法培养肾小球系膜细胞(MC)、转种培养后分为二组,诱导组加LPS10mg/L,对照组不加,用分子生物学和免疫学方法进行检测。结果:IL┐6在正常MC中分泌量极微,其mRNA也为低水平表达,经LPS诱导后二者均明显增加。IL┐6蛋白的产生6~12h达高峰,比对照组增加3~5倍,IL┐6mRNA的表达3~6h达高峰,较对照组增加2~3倍。结论:LPS可诱发系膜细胞IL┐6水平增高,可能作为炎症介质参与肾脏疾病的发病。
nthisstudy,IL-6productionandgeneexpressionwereinvestigatedinculturedmesangialcelswithorwithoutthestimulationofLPS.METHODSIL-6productioninculturedSDratmesangialcels(MCs)wasmeasuredbyELISA,andIL-6mRNAexpressiondetectedbyNorthernblotassay.ComparisonwasmadebetweennormalmesangialcelsandLPSco-culturedmesangialcels.RESULTSTheproductionandmRNAexpressionofIL-6inMCsstimulatedbyLPSweresignificantlyin-creasedascomparedwithnormalcontrol.TheproteinproductionofIL-6reachedpeakat6~12hours,whichwas3~5timeshigherinLPSstimulatedMCsthaninnormalMCs.PeaklevelofIL-6mRNAexpressionap-pearedat3~6hoursandwas2~3timeshigherinLPSstimulatedMCsthaninnormalMCs.CONCLUSIONLPSpotentiatestheefectofIL-6ontheproliferationofMCsinculture.IL-6playsaroleinthepathogenesisofdevelopmentofMsPGN.
出处
《肾脏病与透析肾移植杂志》
CAS
CSCD
1996年第5期7-9,共3页
Chinese Journal of Nephrology,Dialysis & Transplantation
基金
国家卫生部科研基金
CMB基金