摘要
目的:考察两种载肽聚乳酸((polylatic acid,PLA)免疫微球M1(hAFP158~166)和M2(hAFP218~226)在体外诱导特异性CTL的能力及其对肝癌细胞的杀伤作用。方法:制备分别荷载表位肽hAFP158~166、hAFP218~226的PLA免疫微球M1和M2,体外刺激HLA—A2^+健康志愿者的外周血单个核细胞(PBMC)作为效应细胞,实验分为3组:对照组、hAFP表达组和hAFP阴性组。采用标准^(51)Cr释放法检测CTL杀伤活性。结果:两种免疫微球在体外均能刺激人PBMC增殖,形成大量可见克隆;两者诱导的效应细胞对hAFP^+的荷肽他细胞、HepG-2和Alexander的杀伤率均达75%以上,均显著高于不表达hAFP的膀胱癌细胞BTT和未荷肽的他细胞,差异非常显著(P<0,01),而两者杀伤活性之间没有明显差异(P>0.05)。结论:两种载肽聚乳酸免疫微球均能在体外诱导产生特异性CTL,并对表达靶抗原的肝癌细胞有较强杀伤作用。
Objective: To investigate the in vitro efficiency of two kinds of polylatic acid (PLA) immunomicrospheres :M1 ( hAFP158 ~ 166 ) and M2 ( hAFP218 ~ 226) in specific inducement of CTL and the cytotoxicity of CTL against hepatocellular carcinoma ceils. Methods: Pripheral blood monocytes (PBMC) of HLA-A2 healthy volunteers stimulated in vitro by peptide-loading microspheres were taken as effectors. The experiments were divided into three groups: control group, hAFP^+ tumor ceils group, and hAFP- tumor ceils group. The specific cytolysis of target ceils was assessed by ^(51)Cr-release assay. Results: Both M1 and M2 induced proliferation of HLA-A2^+ PBMC, forming visible clones. The effector ceils induced by M1 and M2 both had an cytolysis rate over 75% for hAFP^+ tumor ceils, which was significantly higher than that for hAFP-tumor cells (P〈0. 01 ). But there was no significant difference in cytolysis rate concerning the two kinds of microspheres (P〉0. 05 ). Conclusion: The two kinds of microspheres can both induce specific CTL in vitro, which can effectively kill hAFP^+ tumor ceils.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2006年第3期167-170,共4页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金资助项目(N0.30070855)
关键词
肝癌细胞
聚乳酸微球
AFP
CTL表位肽
Hepatocellular carcinoma cell
PLA microspheres
AFP
CTL epitope peptide