磁性聚乳酸羟基乙酸氧化酚砷纳米微粒对人肝癌细胞生长的抑制作用

Magnetic poly D,L-lactide-co-glycolide phenylarsine oxide nano-particles inhibits hepatic carcinoma

目的:研究磁性聚乳酸羟基乙酸氧化酚砷纳米微粒对人肝癌细胞生长的抑制作用。方法:运用超声乳化-溶剂挥发法制备磁性聚乳酸羟基乙酸氧化酚砷纳米微粒;透射电镜观察纳米微球形态;MTT法检测纳米微粒对肝癌细胞SMMC-7721生长的抑制作用;流式细胞术检测细胞凋亡。结果:磁性聚乳酸氧化酚砷纳米微粒外观呈规则球型,其粒径尺寸平均为290nm。磁性纳米微粒抑制人肝癌细胞增殖,且具有一定的时间和浓度依赖性。1.0μmol/L纳米微粒组作用24h肝癌细胞生长的抑制率为(4.6±0.9)%,作用48h抑制率为(11.4±1.2)%。流式细胞术检测可见肝癌细胞凋亡峰出现,细胞周期阻滞在S+G2/M期。结论:磁性聚乳酸羟基乙酸氧化酚砷纳米微粒具有抑制肝癌细胞SMMC-7721增殖的作用,处于G0/G1期的SMMC-7721细胞可能为其药物作用的目标。

Objective： To study the inhibitory effects of magnetic poly D, L-lactide-co-glycolide phenylarsine oxide nanoparticles （M-PLGA-PAO-NS） on the growth of human hepatic carcinoma cells. Methods： The magnetic poly D,L- lactide-co-glycolide phenylarsine oxide nano-particles were prepared by emulsion-evaporation process. The growth inhibition of tumor cell was studied by the microscope observation and MTF method, and cell apoptosis was observed by FCM. Results： The prepared M-PLGA-PAO-NS had a regular spherical surface and a mean diameter of 290 nm. The magnetic nano-particles effectively inhibited the growth of carcinoma cells （ SMMC-7721 ） in a time- and concentration-dependent manner [ e. g. inhibitory rate of 1.0 μmol/L nano-particles （4.6 ±0.9） % for 24 h versus （ 11.4 ± 1.2） % for 48 h ]. FCM assay indicated that most of the cells were arrested at S ＋ G2/M phase and there was apoptotic peak . Conclusion： The prepared M-PLGA-PAO-NS has high anti-hepatic carcinoma activity against SMMC-7721, and cells in the G0/G1 phase may be the target for drug action.