白细胞介素24对人树突状细胞活化和成熟的诱导作用

Interleukin-24 induced activation and maturation of human dendritic cells

目的:研究白细胞介素24(interleukin-24,IL-24)对人外周血单核细胞来源树突状细胞(dendriticcell,DC)表型及抗原提呈功能的影响。方法:利用Westernblot检测DC培养上清中IL-24的分泌水平;利用半定量RT-PCR方法分析不同条件下DC表达IL-24受体及趋化因子受体的情况;通过流式细胞术分析检测IL-24共培养后DC细胞表面CD80、CD86、MHCⅡ类分子的表达水平;采用体外混合淋巴细胞实验分析经IL-24共培养对DC抗原提呈功能的影响。结果:体外培养过程中,用LPS刺激DC可诱导其分泌IL-24;同时,LPS还能上调DC表达IL-24受体亚单位IL-22R1。IL-24作用于DC可上调DC细胞表面CD80、CD86及MHCⅡ类分子的表达,并增强DC对T细胞的抗原提呈功能。结论:IL-24这一新型的细胞因子在体外实验中能促进DC的表型成熟,增强DC的抗原提呈功能。

Objective： To investigate the effects of human IL-24 on phenotype and antigen presenting function of human monocyte-derived dendritic cells in vitro. Methods： Western blot was used to determine the level of IL-24 in the DC culture supernatant. Semi-quantitative RT-PCR was used to analyze the expression of IL-24 receptor and chemokine receptors in DCs at different developmental stages. Fluorescence-activated cell sorting （FACS） analysis was used to assess the expression of MHC class Ⅱ molecule, CD80 and CD86 on DCs surface in the presence of IL-24. Allogenic mixed lymphocyte reaction was employed to analyze the effect of IL-24 on the antigen presenting function of DCs. Results： We found that LPS induced DCs to secrete endogenous IL-24. Meanwhile, LPS also up-regulated the expression of IL-22R1, an IL- 24 receptor subunit, on DCs. Supplement of IL-24 to DCs culture medium significantly augmented the expression of MHC Ⅱ , CD80, and CD86 on DCs surface, and promoted the antigen presenting function of DCs to T cells. Conclusion： IL- 24 can promote the activation and maturation of DCs, increasing the antigen presenting function of DCs.