人主动脉硫酸乙酰肝素蛋白聚糖对胎儿主动脉平滑肌细胞c－fos和c－myc基因表达的影响

The Effect Of Human Aortic Heparan Sulfate Proteoglycan on the Expression of c-fos and c-myc Gene in the Cultured Fetal Aortic Smooth Muscle Cells

本室以往的研究发现硫酸乙酰肝素蛋白聚糖对培养的胎儿主动脉平滑肌细胞的增殖有明显的抑制作用。本实验采用Ｎｏｒｔｈｅｒｎｂｌｏｔ的方法观察硫酸乙酰肝素蛋白聚糖对胎儿主动脉平滑肌细胞的ｃ－ｆｏｓ，ｃ－ｍｙｃ原癌基因的表达的影响，发现前者能抑制后者的表达。因此硫酸乙酰肝素蛋白聚糖抑制平滑肌细胞增殖的途径之一可能为抑制原癌基因的表达。

Our laboratory have found that human aortic heparan sulfate proteoglycan(HSPG) can suppress the proliferation of cultured fetal aortic smooth muscle cells(hASMC).In this study Norther blot was used to invetigate the effect of HSPG on the expression of c-fos and c-myc proto-oncogene in hASMC. The results showed that former can suppress both c-fos and c-myc gene. It is concluded that suppression of protooncogene expression may be one of the causes that HSPG suppresses smooth muscle cell proliferation.