用HPLC法和氨基酸分析仪测定多维氨基酸片中18种氨基酸

Determination of 18 kinds of amino acids in multi-amino-acid tablets by RP-HPLC method and amino acid analyzer

目的：RP-HPLC法和氨基酸分析仪（amino acid analyzer，AAA）法测定氨基酸含量的比较。方法：采用Agilent高效液相色谱系统和日立835-50氨基酸分析仪测定多维氨基酸片中18种氨基酸的含量。RP-HPLC法采用C18柱和邻苯二甲醛柱前衍生化进行，AAA法采用离子交换色谱柱和茚三酮柱后衍生化进行。结果：HPLC法和AAA法的变异系数分别〈1．5％和3％，最小检测量分别为3pmol和30pmol。HPLC法的测定值与AAA的测定值有相关性。两种测定方法的平均相对偏差为5．74％（0．24％～9．60％）。结论：两种方法都可用于测定氨基酸，但测定结果存在差异。

Objective： To compare the methods of RP-HPLC and amino acid analyzer（AAA） for determining concentrations of amino acids. Methods： Eighteen kinds of amino acids in multi-amino-acid tablets were measured by an Agilent HPLC and Hitachi 835-50 amino acid analyzer. One C18 column was used for HPLC method with o-pathalaldehyde precolumn derivation. An ion exchange chromatography column was used for AAA method with ninhydrin postcolumn derivation. Results： The coefficients of variation of HPLC and AAA methods were less than 1.5 % and 3.0%, respectively. The minimal detectable limit was 3 pmol for HPLC method and 30 pmol for AAA method. The results obtained from RP-HPLC were correlated to those of AAA method. The average relative standard deviation of the two methods was 5.74% （ranged from 0.24% to 9.60 %）. Conclusion： Both methods can be used to determine the concentrations of amino acids, but there is difference between the results of the two methods.