应用GC-MS检验羊肉中阿托品的方法研究

Detection of Atropine in Mutton with Gas Chromatograph-Mass Spectrometer

目的研究应用气相色谱-质谱联用仪测定羊肉中阿托品的方法，为此类检测提供依据。方法用酸性水萃取，经过滤处理，甲醇溶解，气相色谱-质谱联用仪测定；色谱条件：DB-5MS毛细管色谱柱，程序升温方式升温，起始温度100℃保持1min，以15℃／min速率升温至280℃保持2min；质谱条件：离子阱温度150℃，传输线温度200℃，歧管温度45℃，电离方式EI（电子轰击），轰击能量70eV，特征离子质量数42、82、124（M／Z）。结果本法最低检测限为20ng，平均回收率83％，相对标准差RSD11．6％，线性范围0～100ng。结论本方法适合于食物中毒样品中阿托品的测定。

Objective In order to establish a method for determination of atropine in mutton. Methods The samples were purified by acidity water, dissolved by methyl alcohol, and then analyzed with GC - MS; The conditions for the GC were a DB -5MS capillary column, and the programming temperatures： initial temperatures were 100℃, hold lmin, rate of heating 15℃/min, from 100℃ to 280℃, hold 2min; The conditions for the MS were that the temperature of the ion trap was 150℃, the temperature of the transfer line was 200℃, the temperature of the manifold was 45℃, the module option key was EI, the energy was 70eV, and the characteristic ions were 42,82 and 124（M/Z）. Results The detection limit of the method was 20ng, the recovery rate was 83%, and the RSD was 11.6%. The range of linearity was 0 - 100ng. Conclusions It was concluded that the method is suitable for determination of atropine in mutton.