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Characteristics of transgenic tomatoes antisensed for the ethylene receptor genes LeERT1 and LeERT2

Characteristics of transgenic tomatoes antisensed for the ethylene receptor genes LeERT1 and LeERT2
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摘要 Two stable transformed lines containing antisense LeERT1 or LeERT2 sequences and their hybridized line were investigated to determine the effect of LeERT1 and LeERT2 specificity in the ethylene receptor family in tomato (Lycopersicon esculenturn Mill.) on ethylene signaling. The transgenic line alel containing antisense LeER T1 displayed shorter length of seedling grown in the dark and adult plant in the light, severe epinastic petiole, and accelerated abscission of petiole explant and senescence of flower explant, compared with its wild type B 1. The transgenic line ale2 containing antisense LeERT2 also exhibited shorter hypocotyls and slightly accelerated abscission. The phenotypes of cross line dale of LeERT1 and LeERT2 were close to alel in many aspects. These results suggested that LeERT1 probably plays a relatively important role in ethylene signaling of tomato growth and development. Two stable transformed lines containing antisense LeERT1 or LeERT2 sequences and their hybridized line were investigated to determine the effect of LeERT1 and LeERT2 specificity in the ethylene receptor family in tomato (Lycopersicon esculentum Mill.) on ethylene signaling. The transgenic line ale1 containing antisense LeERT1 displayed shorter length of seedling grown in the dark and adult plant in the light, severe epinastic petiole, and accelerated abscission of petiole explant and senescence of flower explant, compared with its wild type B1. The transgenic line ale2 containing antisense LeERT2 also exhibited shorter hypocotyls and slightly accelerated abscission. The phenotypes of cross line dale of LeERT1 and LeERT2 were close to ale1 in many aspects. These results suggested that LeERT1 probably plays a relatively important role in ethylene signaling of tomato growth and development.
出处 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2006年第7期591-595,共5页 浙江大学学报(英文版)B辑(生物医学与生物技术)
基金 Project (No. 30371001) supported by the National Natural ScienceFoundation of China
关键词 Antisense transformation Ethylene receptor Ethylene response TOMATO 乙烯受体 乙烯响应 西红柿 反义转换 LeERT1 LeERT2
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参考文献2

  • 1Dingbo Zhou,Panagiotis Kalaitzis,Autar K. Mattoo,Mark L. Tucker.The mRNA for an ETR1 homologue in tomato is constitutively expressed in vegetative and reproductive tissues[J].Plant Molecular Biology.1996(6)
  • 2Peter Hajdukiewicz,Zora Svab,Pal Maliga.The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation[J].Plant Molecular Biology.1994(6)

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