Survivin在涎腺良性、交界性、恶性多形性腺瘤中的表达及意义

The expression of survivin gene in malignant pleomorphic adenoma,relay pleomorphic adenoma and pleomorphic adenoma

目的探讨Survivin在涎腺良性多形性腺瘤(PA)、交界性多形性腺瘤(RPA)、恶性多形性腺瘤(MPA)中的表达及其与肿瘤增殖活性的相关性。方法应用免疫组织化学方法及原位杂交技术检测14例涎腺PA、9例RPA、11例MPA以及9例癌旁腺组织中Survivin的表达。结果Survivin mRNA在涎腺PA、RPA、MPA中的表达与其蛋白表达相比均无显著性差异,而在癌旁腺体中的表达存在显著性差异(P<0.05);在涎腺PA中Survivin mRNA及其蛋白的表达均明显低于RPA、MPA及癌旁组织(P<0.01),而RPA与MPA中的表达无显著性差异;在MPA中高分化者与中低分化者的表达均有显著性差异(P<0.05)。结论Survivin基因的转录和扩增可能参与多形性腺瘤的发生、发展及恶变进程,并且在肿瘤发生的不同阶段有不同表达,有望成为涎腺肿瘤诊断和治疗的新靶点。

Objective To study the expression of survivin in malignant pleomorphic adenoma, relay pleomorphic adenoma and pleomorphic adenoma. Methods The transcription and interpretation of urvivin mRNA in the salivary gland tumor were analyzed via immunohistochemistry and in situ hybridization methods. Results Survivin mRNA expression and protein expression were almost the same in malignant pleomorphic adenoma, relay pleomorphic adenoma and pleomorphic adenoma. But they were different in the gland around the malignant pleomorphic adenoma. There was a significant difference of Survivin mRNA and protein expression in pleomorphic adenoma and malignant pleomorphic adenoma, and relay pleomorphic adenoma. The expressions were almost the same in relay and malignant pleomorphic adenoma. Conclusion The expression of survivin mRNA and protein is increased in pleomorphic adenoma and may be involved in the onset and progression of pleomorphic adenoma. It indicates that mRNA is relatively stable in the mRNA transcription and protein interpretation in malignant pleomorphic adenoma, and it is also showed earlier in gene amplification than in protein expression of the malignant tumor in the initial stage of malignant pleomorphic adenoma. Survivin can be a new diagnostic/therapeutic target in pleomorphic adenoma.