b-FGF和TGF-β1对体外培养的软骨细胞增殖的影响

Experimental research on proliferation of chondrocytes in vitro using TGF-β1 and/or b-FGF

目的:研究转化生长因子-β1(TGF-β1)和碱性成纤维细胞生长因子(b-FGF)对体外培养的软骨细胞增殖的影响。方法:细胞取自猪耳弹性软骨,体外培养原代至第6代软骨细胞,将培养液中加入TGF-β1或/和b-FGF作用于软骨细胞,并用MTT比色分析法对其增殖行为进行分析,探讨两种因子对软骨细胞增殖行为的影响。结果:b-FGF明显促进原代至第3代软骨细胞的增殖,并逐渐减弱,对第4代细胞无增殖作用,TGF-β1仅促进原代软骨细胞的增殖,TGF-β1和b-FGF联合应用,其刺激细胞增殖的作用明显低于b-FGF单一作用。结论:b-FGF显著促进体外培养的软骨细胞的增殖,主要作用于原代至第2代软骨细胞,明显强于b-FGF和TGF-β1联合应用。

Objective To study the effect of proliferation of porcine elastic chondrocytes in vitro using TGF-β1 and/ or b-FGF. Methods Cells were harvested from the elastic auricular cartilage of swines. To study the rule of proliferation of chondrocytes from passage 0 to passage 6 in Ham＇s F-12 media including TGF-β1 and/or b-FGF Using MTT method, Results b-FGF had an signif icant effect on proliferation of chondrocytes from passage 0 to passage 3,which disappeared in passage 4. TGF-β1 had an increasing effect on chondrocytes only in passage 0.The combination of TGF-β1 and b-FGF led to a less effect on proliferation of chondrocytes than b-FGF, Conclusion b-FGF had a significantly increasing effect on proliferation of chondrocytes from passage 0 to passage 3, which is more signicant than the effect of combination of TGF-β1 and b-FGF.