HPLC法测定复方沙棘籽油栓中蛇床子素的含量

Determination of osthole in Compound Shajiziyou Suppositories by HPLC

目的测定复方沙棘籽油栓中主要成分蛇床子素的含量。方法采用HPLC法,C8色谱柱;流动相为甲醇-水(75∶25);流速0.7 mL.min-1;检测波长320 nm;柱温30℃;进样量为10μL。结果蛇床子素含量与峰面积线性关系良好,回归方程为A×10-3=-7.950+7.384 X,r=0.999 5(n=5),平均回收率为98.3%,RSD值为0.71%。结论HPLC法测定复方沙棘籽油栓中蛇床子素的含量可行。

Objective To determine the contents of osthole in Compound Shajiziyou Suppositories. Methods The HPLC conditions were as follows： Cscolumn, methanol-water （75 ： 25） as mobile phase, 0. 7 mL · min^-1 as flow-rate and 320nm as detective wavelength; The column temperature was 30℃ ;The volume of injection was 10 μL. Results This method had a good linearity between osthole concentration and peak area. The linear equation wasA×10^-3=-7.950＋7.384X,r=0.9995（n=5）. The average recovery was 98.3%, with RSD 0.71%. Conclusion This method can be used to determine osthole in Compound Shajiziyou suppositories.