35例维吾尔族妇女宫颈癌组织中HPV16型E7致癌基因突变分析

Human Papillomavirus 16 E7 Oncogene Variants in Xinjiang Uygur Women with Cervical Carcinoma

目的本研究旨在检测HPV16E7基因在新疆南部维吾尔妇女宫颈癌组织中的突变。方法从35份新疆南部地区维吾尔族妇女宫颈癌活检组织标本中提取组织DNA,作为模板,PCR扩增HPV16 E7全长基因,PCR产物直接测序或克隆后测序,分析新疆维吾尔族妇女宫颈癌组织HPV16E7基因的突变。结果PCR检测结果宫颈癌组织中HPV16E7阳性率为82.86%(29/35);27个E7分离片段的测序和序列分析表明,26个分离株E7基因与原型相同,1个分离株E7基因核苷酸发生2处突变,即647位(在HPV16基因组中的位置)的A→G,氨基酸由Asn变异为Ser;845位的T→C,氨基酸不变。结论新疆南部地区维吾尔妇女宫颈癌患者感染的HPV16中存在E7基因的变异株,该变异株可能是由人口流动传入。

Objective This study was designed to investigate the variant of HPV16 E7 from cervical carcinoma biopsies in Xinjiang Uygur Women. Methods The tissue DNA was extracted from 35 cervical carcinoma biopsies in Xinjiang Uygur Women. HPV16E7 genes were amplified by PCR from the cervical carcinoma tissue DNA. Nucleotide sequences of the HPV16 E7 genes were determined with direct or cloning sequencing methods to analyze the mutations of HPV16 type E7 gene. Results The result of PCR showed that the positive rate of HPV16 E7 was 82.86% （ 29/35 ）. The DNA was sequenced. By analyzing the mutations of 27 E7 gene from 29 cases, we found 26 HPV16 E7 among the 27 cases were maintained to be prototype, two mutations were detected in one E7 gene among the 27 cases, one was 647 （ A→G） , resulting in amino acid Ser substituted for Ash ; the other was 845 （ T→C ） , without variation of amino acids. Conclusions There is a variant strain of HPV16 E7 gene from cervical carcinoma biopsies in the Uygur Women from southern Xinjiang, the variant strain might be instructed into the region by population floating.